BIOSYNTHESIS OF POLYUNUNSATORATED FATTY ACIDS ON THE BASIS OF CHLORELLA SOROKINIANA MICROALGAE

Abstract

The effect of the cultivation conditions of the microalgae Chlorella sorokiniana on the synthesis of polyunsaturated fatty acids was studied.  Among all strains of Chlorella, Chlorella sorokiniana is the most suitable for the extraction of omega (ω)-3 and ω-6 polyunsaturated fatty acids. The rich composition of lipids makes it possible to effectively use it as a biological mixture of polyunsaturated fatty acids, in particular eicosapentaenoic and docosahexaenoic acids. Parameters of realization of effective biosynthesis docosahexaenoic acid with an output of a biomass are found, at corresponding optimum values of temperature (25 – 27^oС), pН (7,7-8,0), illumination (2000-3000 lux) and speed of hashing (20 revolutions per minute). The effect of the cultivation conditions of the microalgae Chlorella sorokiniana on the synthesis of polyunsaturated fatty acids was studied. The results of the analysis  of higher fatty acids (with more than 16 carbon atoms) show the presence of unsaturated fatty acids C18:1, C18:2, C18:3, C20:3, C20:4, C20:5, C22:6 with a predominance of polyunsaturated fatty acids in the form of omega-3 (eicosopentaenoic,  docosahexaenoic, linolenic acids).